The new 2-year fixed rates include 4.19%, reduced from 5.29% at 75% loan to value and 3.69%, reduced from 3.88 per cent, at 60% LTV.
The new range reverts to a lifetime tracker of base plus 4.49%.
Andy Gray, managing director of mortgages at Barclays, said: “We know that 2- year fixed rates requiring a 25% deposit have been proving immensely popular with investors and landlords looking for certainty with their mortgage repayments.
"We’ve slashed the rate for those borrowers with a smaller deposit making it the best deal we’ve offered at 75% LTV since we launched in November last year.
"These new products will help customers to save money and make investment in the buy-to-let market more affordable.”
Gray added: "The buy-to-let mortgage market has continued to grow over recent years and is fiercely competitive so we expect that these rates cuts will be welcomed by landlords.”